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peptide-mapping

Page 5 of 5 · 103 questions tagged peptide-mapping.

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103 questions · sorted by votes · page 5 of 5

4 votes 2 answers3.1k views
Is 98.8% a realistic purity ceiling for liraglutide given its chain length?

Conditions: 98.8% · liraglutide. Please show the division. I want to check my own against yours. I would like the general form as well as the specific number, so I…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questionshplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questionspeptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

103 questionsliraglutide
liraglutide

A once-daily GLP-1 receptor agonist and the compound that established the class. Still relevant for its shorter half-life, its paediatric and…

235 questionsasked by retest_please 9.7k · 12 months ago
3 votes5 answers9.9k views
Is 99.4% a realistic purity ceiling for ecnoglutide given its chain length?

The particulars: 99.4% · ecnoglutide. I would rather understand the derivation than memorise the outcome. Two people I asked gave two answers that differ by a factor…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questionshplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questionspeptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

103 questionsecnoglutide
ecnoglutide

A long-acting GLP-1 receptor agonist with a cAMP-biased signalling profile. A niche tag, mostly used for mechanism questions about biased agonism…

223 questionsasked by dead_volume 56k · 10 months ago
3 votes3 answers9.4k views
How should I budget for peptide mapping across a twelve-month ecnoglutide course?

What I am working with: peptide mapping · ecnoglutide. I would like to define my thresholds before I have a result, for obvious reasons. I want a plan with explicit…

batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

910 questionscost-analysis
cost-analysis

Cost arithmetic done honestly: cost per milligram after dead-space loss, list versus net price, comparing a multi-dose vial to a fixed-dose pen,…

260 questionsjanoshik
janoshik

Janoshik Analytical, an independent third-party testing laboratory widely used by this community for HPLC purity, identity and quantified content…

139 questionspeptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

103 questionsecnoglutide
ecnoglutide

A long-acting GLP-1 receptor agonist with a cAMP-biased signalling profile. A niche tag, mostly used for mechanism questions about biased agonism…

223 questionsasked by cap_the_luer 14k · 13 months ago
3 votes 5 answers6.4k views
How many vials from an FGP lot should I send for peptide mapping?

Stated plainly: FGP · peptide mapping. The units are where I keep going wrong, so please be explicit about them. I have sanity-checked the order of magnitude and it…

batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

910 questionscoa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questionscost-analysis
cost-analysis

Cost arithmetic done honestly: cost per milligram after dead-space loss, list versus net price, comparing a multi-dose vial to a fixed-dose pen,…

260 questionspeptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

103 questionsasked by drawn_and_capped 12k · 5 months ago
2 votes 5 answers19k views
Is 97.1% a realistic purity ceiling for liraglutide given its chain length?

Concretely: 97.1% · liraglutide. I want the working, not the result — I need to be able to redo it with different numbers. I care about the precision as well as the…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questionshplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questionspeptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

103 questionsliraglutide
liraglutide

A once-daily GLP-1 receptor agonist and the compound that established the class. Still relevant for its shorter half-life, its paediatric and…

235 questionsasked by thermal_mass 13k · 2.3 years ago
1 vote 4 answers5.7k views
Is 98.4% a realistic purity ceiling for a GLP-1 receptor agonist given its chain length?

What I am working with: 98.4% · a GLP-1 receptor agonist. I have worked this out and I would like someone to find the error, because I suspect there is one. My…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questionshplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questionspeptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

103 questionsasked by Dr_Ravi_Selvarajah 35k · 9 months ago
1 vote0 answers1.4k views
Would a Karl Fischer water content test distinguish deamidation from a synthesis impurity in dulaglutide?

Conditions: a Karl Fischer water content test · deamidation · dulaglutide. I would like the axes of comparison first and the recommendation second. I have tried the…

peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

103 questionspurity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questionsmass-spec
mass-spec

Mass spectrometry for identity confirmation: electrospray ionisation, multiple charge states, monoisotopic versus average mass, deconvolution, and…

52 questionswater-content
water-content

Residual moisture in a lyophilised solid, measured by Karl Fischer titration or loss on drying. It drives both the mass balance on a content assay…

51 questionsdulaglutide
dulaglutide

A once-weekly GLP-1 receptor agonist built on an Fc fusion rather than fatty-acid acylation. Use this tag for questions about the fusion-protein…

200 questionsasked by mira_sundqvist 7.1k · 10 months ago